期刊
RSC ADVANCES
卷 6, 期 30, 页码 25427-25434出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra27563f
关键词
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资金
- special fund of Specialized Research Fund for the Doctoral Program of Higher Education [20130094120009]
- National Natural Science Foundation of China [51308183, 51179052]
- Natural Science Foundation of Jiangsu Province of China [BK20130828]
- Fundamental Research Funds for the Central Universities [2013B32214, 2014B07414, 2015B36414]
- Jiangsu Province Ordinary University Graduate Student Scientific Research Innovation Project [KYLX15_0475]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
A highly efficient enzyme-based screen printed electrode (SPE) was developed based on the covalent immobilization of tyrosinase (Tyr) on 1-formylpyrene (1-FP) functionalized reduced graphene oxide (rGO). Here, the bifunctional molecule 1-FP was assembled onto rGO sheets. Subsequently, a Tyr molecule was immobilized on the 1-FP forming a biocompatible nanocomposite, which was further coated onto the working electrode surface of the SPE. The performance of as-prepared biosensor was investigated by the detection of phenol in the presence of molecular oxygen. Wide linear range, low detection limit and high sensitivity were obtained with this biosensor due to the good conductivity of rGO as well as the high bioactivity of Tyr well retained by the 1-FP/rGO platform. Finally, the proposed biosensor was successfully employed for the detection of phenol in real water samples with satisfactory results. These findings suggest that this novel biosensor could offer great potential for rapid, costeffective and on-field analysis of phenolic compounds.
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