期刊
RSC ADVANCES
卷 6, 期 56, 页码 51367-51373出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ra09568b
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资金
- Karolinska Institute
- Swedish Research Council
- Polish National Center of Research and Development [02/EuroNanoMed/2011]
- The National Science Centre (Poland) [UMO-2012/05/E/ST5/03893]
Achieving higher nuclear concentrations by active transport may give potent therapeutic effects at lower doses for many drugs. A method of increasing nuclear uptake is the use of naturally existing Nuclear Localization Signals (NLS) by conjugating NLS structures to the cargo. We have synthesized a set of 2,2,7-trimethylguanosine cap (m(3)G-CAP)-containing structures (and their biotin conjugates) as artificially attached analogs of a naturally found NLS. The origin of a naturally found NLS is a uridine rich, small nuclear ribonucleoprotein (U snRNP) that employs Snurportin1 as a nuclear transport protein. In this report the NLS activity of various m(3)G-CAP biotin constructs was studied. We have shown that a minimal requirement for nuclear uptake is the inclusion of a trinucleotide sequence between the m3G-CAP and the artificial linker.
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