期刊
RSC ADVANCES
卷 6, 期 83, 页码 79798-79804出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6ra16145f
关键词
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资金
- National Natural Science Foundation of China [21465014, 21465015]
- Natural Science Foundation of Jiangxi Province [20142BAB203101]
- Ministry of Education by Specialized Research Fund for the Doctoral Program of Higher Education [20133604110002]
- Ground Plan of Science and Technology Projects of Jiangxi Educational Committee [KJLD14023]
- Open Project Program of Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University [KLFS-KF-201410, KLFS-KF-201416]
In this work, a novel hydrogen peroxide (H2O2) electrochemical biosensor was developed based on the metal-organic framework (MOF, Tb@mesoMOF) modified three-dimensional (3D) kenaf stem-derived macroporous carbon (3D-KSCs) electrode which was used as a new platform to load microperoxidase-11 (MP-11) for the first time. The chitosan-Au nanoparticles (CHIT-AuNPs) were firstly electrodeposited on the 3D-KSCs integrated electrode, which provided a large number of active sites for the growth of Tb@mesoMOFs to form the Tb@mesoMOFs/CHIT-AuNPs/3D-KSCs integrated electrode. Then the MP-11 molecules were effectively encapsulated in Tb@mesoMOFs of the Tb@mesoMOFs/CHIT-AuNPs/3D-KSCs integrated electrode. Scanning electron microscopy and energy disperse X-ray spectroscopy was used to characterize the resulting electrode. The results showed that a large number of spherical Tb@mesoMOFs grew on the CHIT-AuNPs/3D-KSCs electrode uniformly and firmly. The electrochemical performance of the MP-11/Tb@mesoMOFs/CHIT-AuNPs/3D-KSCs electrode were also explored. The resulting MP-11/Tb@mesoMOFs/CHIT-AuNPs/3D-KSCs integrated electrode showed good performance toward the detection of H2O2 with a wide linear range from 3.02 mu M to 640 mu M and a low detection limit of 0.996 mu M. This work provides new ideas to design MOFs/3D-KSCs integrated electrodes for enzyme immobilization to construct electrochemical biosensors.
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