期刊
ONCOTARGET
卷 7, 期 18, 页码 26070-26086出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.8429
关键词
lung adenocarcinoma; miRNA; plasma; diagnosis; biomarker
资金
- National Basic Research Program of China (973program) [2012CB9333004]
- Science and Technology Department Fund Project Affiliated with the Education Department of Tianjin [20100119]
Background: The subtypes of NSCLC have unique characteristics of pathogenic mechanism and responses to targeted therapies. Thus, non-invasive markers for diagnosis of different subtypes of NSCLC at early stage are needed. Results: Based on the results from the screening and validation process, 3 miRNAs (miR-532, miR-628-3p and miR-425-3p) were found to display significantly different expression levels in early-stage lung adenocarcinoma, as compared to those in healthy controls. ROC analysis showed that the miRNA-based biomarker could distinguish lung adenocarcinoma from healthy controls with high AUC (0.974), sensitivity (91.5%), and specificity (97.8%). Importantly, these three miRNAs could also distinguish lung adenocarcinoma from lung benigh diseases and other subtypes of lung cancer. Methods: Two hundreds and one early-stage lung adenocarcinoma cases and one hundreds seventy eight age-and sex-matched healthy controls were recruited to this study. We screened the differentially expressed plasma miRNAs using TaqMan Low Density Arrays (TLDA) followed by three-phase qRT-PCR validation. A risk score model was established to evaluate the diagnostic value of the plasma miRNA profiling system. Conclusions: Taken together, these findings suggest that the 3 miRNA-based biomarker might serve as a novel non-invasive approach for diagnosis of early-stage lung adenocarcinoma
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