期刊
ONCOTARGET
卷 7, 期 7, 页码 8223-8239出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.6972
关键词
invasion; microRNA (miRNA); epithelial-mesenchymal transition (EMT); head and neck squamous cell carcinoma (HNSCC); microarray
资金
- JSPS KAKENHI [23689074, 23659858, 21249088]
- Research Fellowship for Young Scientists from the Japan Society for the Promotion of Science [23-6562]
- Grants-in-Aid for Scientific Research [26290047, 16H05164, 23689074, 21249088, 23659858] Funding Source: KAKEN
Head and neck squamous cell carcinoma (HNSCC) has a high capacity for invasion. To identify microRNAs (miRNAs) that regulate HNSCC invasion, we compared miRNA expression profiles between a parent HNSCC cell line and a highly invasive clone. The miR-200 family and miR-203 were downregulated in the clone. Here we focused on the role of miR-203 in invasion and epithelial-mesenchymal transition (EMT) induction in HNSCC. miR-203 was downregulated during EMT induction. Moreover, ectopic overexpression of miR-203 suppressed the invasion and induced mesenchymal-epithelial transition (MET) in HNSCC cells. Interestingly, we identified NUAK family SNF1-like kinase 1 (NUAK1) as a novel target gene of miR-203 by cyclopedic analysis using anti-Ago2 antibody. Increased expression of NUAK1 was observed during EMT induction, and ectopic expression of miR-203 delayed EMT induction by suppressing NUAK1 expression. Moreover, NUAK1 overexpression promoted the invasion of HNSCC cells. Importantly, NUAK1 expression was well correlated with poor differentiation, invasiveness, and lymph node metastasis in HNSCC cases. Overall, miR-203 has a tumor-suppressing role in invasion and EMT induction by targeting NUAK1 in HNSCC, suggesting miR-203 as a potential new diagnostic and therapeutic target for the treatment of HNSCC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据