期刊
ONCOTARGET
卷 7, 期 18, 页码 24908-24927出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.8795
关键词
extracellular matrix; type I collagen; aging; cancer; discoidin domain receptor 2; cell proliferation; Gerotarget
资金
- Ligue Contre le Cancer (CCIR Grand-Est)
- FEDER/Emergence CELLnanoFLUO Program (Region Champagne-Ardenne)
- French Ministry of Higher Education and Research
Tumor cells are confronted to a type I collagen rich environment which regulates cell proliferation and invasion. Biological aging has been associated with structural changes of type I collagen. Here, we address the effect of collagen aging on cell proliferation in a three-dimensional context (3D). We provide evidence for an inhibitory effect of adult collagen, but not of the old one, on proliferation of human fibrosarcoma HT-1080 cells. This effect involves both the activation of the tyrosine kinase Discoidin Domain Receptor 2 (DDR2) and the tyrosine phosphatase SHP-2. DDR2 and SHP-2 were less activated in old collagen. DDR2 inhibition decreased SHP-2 phosphorylation in adult collagen and increased cell proliferation to a level similar to that observed in old collagen. In the presence of old collagen, a high level of JAK2 and ERK1/2 phosphorylation was observed while expression of the cell cycle negative regulator p21CIP1 was decreased. Inhibition of DDR2 kinase function also led to an increase in ERK1/2 phosphorylation and a decrease in p21CIP1 expression. Similar signaling profile was observed when DDR2 was inhibited in adult collagen. Altogether, these data suggest that biological collagen aging could increase tumor cell proliferation by reducing the activation of the key matrix sensor DDR2.
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