期刊
ONCOTARGET
卷 7, 期 26, 页码 40830-40845出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.8518
关键词
miRNA; adipocyte; adipogenesis; meta-analysis; obesity
资金
- National Key Basic Research Program of China [2013CB530604]
- National Natural Science Foundation of China [81330067, 81200642, 81270928, 81370964, 81500674]
- Program for Innovative Research Teams of Jiangsu Province [LJ201108]
- National Natural Science Foundation of Jiangsu Province [BK20150082]
- Nanjing Technological Development Program [201104013, ZKX14042, YKK15165]
- Medical Science and technology developmental Foundation
- Nanjing Department of Health [JQX13012]
- Science and Technology Development Fund of Nanjing Medical University [2014 NJMUZD041]
MicroRNAs (miRNAs) have been identified as a new class of regulatory molecules that influence many biological functions, including metabolism, adipocyte differentiation. To determine the role of adipogenic miRNAs in the adipocyte differentiation process, we used microarray technology to monitor miRNA levels in human adipose-derived mesenchymal stem cells (hMSCs-Ad), human stromal vascular cells (SVCs) and differentiated adipocytes. 79 miRNAs were found to be differentially expressed, most of which are located in obesity related chromosomal regions but have not been previously linked to adipocyte differentiation process. A systematic search was made for relevant studies in academic data bases, involving the Gene Expression Omnibus (GEO) ArrayExpress, Pubmed and Embase database. Eight studies on human adipocyte differentiation or obesity were included in the final analysis. After combining our microarray data with meta-analysis of published microarray data, we detected 42 differently expressed miRNAs (meta-signature miRNAs) in mature adipocytes compared to SVCs or hMSCs-Ad. Our study shows meta-signature miRNAs specific for adipogenesis, several of which are correlated with key gene targets demonstrating functional relationships to pathways in BMP signaling pathway, Cell differentiation, Wnt signaling, insulin receptor signaling pathway, MAPK signaling, Cell cycle and lipid metabolic process. Our study shows that the first evidence of hsa-let-7 family, hsa-miR-15a-5p, hsa-miR-27a-3p, hsa-miR-106b-5p, hsa-miR-148a-3p and hsa-miR-26b-5p got a great weight in adipogenesis. We concluded that meta-signature miRNAs involved in adipocyte differentiation and provided pathophysiological roles and novel insight into obesity and its related metabolic diseases.
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