期刊
ONCOTARGET
卷 7, 期 11, 页码 12693-12703出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.7281
关键词
EZH2; RIP-seq; MALAT1; gastric cancer; transcriptional silencing
资金
- State Key Development Program for Basic Research of China [2013CB967402]
- Longhua Medical Project of State Clinical Research Center of TCM in Longhua Hospital [LYTD-21, JDZX2012123]
- National Natural Science Foundation of China [91229123, 81373623]
EZH2, the catalytic component of polycomb repressive complex 2 (PRC2), is frequently overexpressed in human cancers and contributes to tumor initiation and progression, in part through transcriptional silencing of tumor suppressor genes. A number of noncoding RNAs (ncRNAs) recruit EZH2 to specific chromatin loci, where they modulate gene expression. Here, we used RNA immunoprecipitation sequencing (RIP-seq) to profile EZH2-associated transcripts in human gastric cancer cell lines. We identified 8,256 transcripts, including both noncoding and coding transcripts, some of which were derived from cancer-related loci. In particular, we found that long noncoding RNA (lncRNA) MALAT1 binds EZH2, suppresses the tumor suppressor PCDH10, and promotes gastric cellular migration and invasion. Our work thus provides a global view of the EZH2-associated transcriptome and offers new insight into the function of EZH2 in gastric tumorigenesis.
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