期刊
ONCOTARGET
卷 8, 期 8, 页码 13048-13058出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.14369
关键词
non-small-cell lung cancer (NSCLC); exosome; miRNAs; prognosis; biomarker
资金
- National Natural Science Foundation of China [81171903, 81472190, 81370139]
- Chongqing Natural Science Foundation of China [cstc2015jcyjBX0110]
- Discipline Construction and Development Foundation from Xinqiao Hospital, Third Military Medical University
- Foundation for Clinical Research from Xinqiao Hospital, Third Military Medical University [2014YLC13]
Exosomal miRNAs are proposed as excellent candidate biomarkers for clinical applications. However, little is known about their potential roles as prognostic biomarkers in lung cancer. In this study, we explored the prognostic value of plasma exosomal microRNAs (miRNAs) for non-small-cell lung cancer (NSCLC). Using a quantitative polymerase chain reaction (qPCR) array panel, we analyzed 84 plasma exosomal miRNAs in 10 lung adenocarcinoma patients and 10 matched healthy controls. The qPCR array showed 30 aberrantly expressed exosomal miRNAs. Nine candidate miRNAs were selected based on differential expression and previous reports for further evaluating their prognostic roles in 196 NSCLC patients. Elevated levels of exosomal miR-23b-3p, miR-10b-5p and miR-21-5p were independently associated with poor overall survival (with hazard ratio [95% confidence interval]: 2.42 (1.45 - 4.04), P = 0.001; 2.22 (1.18 - 4.16), P = 0.013; 2.12 (1.28 - 3.49), P = 0.003, respectively). When compared to the clinical prognostic variables only model, adding the three exosomal miRNA signatures significantly improved survival predictive accuracy with an increase of time-dependent area under the receiver operating characteristic curve from 0.88 to 0.91 (P=0.015). Our results indicated that plasma exosomal miR-23b-3p, miR-10b-5p and miR-21-5p are promising non-invasive prognostic biomarkers of NSCLC.
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