期刊
ONCOTARGET
卷 8, 期 3, 页码 5629-5637出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.13562
关键词
DNA methylation; bisulfite sequencing; gene regulation; chromatin modeling; imprinting
资金
- National Research Foundation
- Singapore Ministry of Education under its Centres of Excellence initiative
- National Medical Research Council of Singapore [NMRC/BNIG/2035/2015]
- Institut Francais a Singapour [6.10.14]
- New England Biolabs, Inc.
The combination of DNA bisulfite treatment with high-throughput sequencing technologies has enabled investigation of genome-wide DNA methylation at near base pair level resolution, far beyond that of the kilobase-long canonical CpG islands that initially revealed the biological relevance of this covalent DNA modification. The latest high-resolution studies have revealed a role for very punctual DNA methylation in chromatin plasticity, gene regulation and splicing. Here, we aim to outline the major biological consequences of DNA methylation recently discovered. We also discuss the necessity of tuning DNA methylation resolution into an adequate scale to ease the integration of the methylome information with other chromatin features and transcription events such as gene expression, nucleosome positioning, transcription factors binding dynamic, gene splicing and genomic imprinting. Finally, our review sheds light on DNA methylation heterogeneity in cell population and the different approaches used for its assessment, including the contribution of single cell DNA analysis technology.
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