期刊
ONCOTARGET
卷 7, 期 32, 页码 52003-52016出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.10468
关键词
osteopontin splice variants (OPN-SV); osteopontin-a (OPNa); thyroid cancer; migration; invasion
资金
- CNPq PhD Scholarship (National Counsel of Technological and Scientific Development, Brazil)
- Science Without Borders [237322/2012-9]
- FCT
- Portuguese Foundation for Science and Technology [SFRH/BD/87887/2012, SFRH/BD/110617/2015]
- FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE - Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal
- Portuguese funds through FCT - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia e Inovacao in the framework of the project Institute for Research and Innovation in Health Sciences [POCI-01-0145-FEDER-007274]
- project Advancing cancer research: from basic knowledgment to application [NORTE-010145-FEDER-000029]
- Projetos Estruturados de I&D&I - Norte - Programa Operacional Regional do Norte
- Fundação para a Ciência e a Tecnologia [SFRH/BD/87887/2012, SFRH/BD/110617/2015] Funding Source: FCT
Osteopontin (OPN) is a matricellular protein overexpressed in cancer cells and modulates tumorigenesis and metastasis, including in thyroid cancer (TC). The contribution of each OPN splice variant (OPN-SV), named OPNa, OPNb and OPNc, in TC is currently unknown. This study evaluates the expression of total OPN (tOPN) and OPN-SV in TC tissues and cell lines, their correlation with clinicopathological, molecular features and their functional roles. We showed that tOPN and OPNa are overexpressed in classic papillary thyroid carcinoma (cPTC) in relation to adjacent thyroid, adenoma and follicular variant of papillary thyroid carcinoma (fvPTC) tissues. In cPTC, OPNa overexpression is associated with larger tumor size, vascular invasion, extrathyroid extension and BRAF(V600E) mutation. We found that TC cell lines overexpressing OPNa exhibited increased proliferation, migration, motility and in vivo invasion. Conditioned medium secreted from cells overexpressing OPNa induce MMP2 and MMP9 metalloproteinases activity. In summary, we described the expression pattern of OPN-SV in cPTC samples and the key role of OPNa expression on activating TC tumor progression features. Our findings highlight OPNa variant as TC biomarker, besides being a putative target for cPTC therapeutic approaches.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据