Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Targeted molecular therapy has gradually been a potential solution in cancer therapy. Other authors' and our previous studies have demonstrated that phosphoinositide-specific phospholipase. (PLC gamma) is involved in regulating tumor growth and metastasis. However, the molecular mechanism underlying PLC gamma-dependent tumor growth and metastasis of gastric adenocarcinoma and whether PLC gamma may be a potential target for tumor therapy in human gastric adenocarcinoma are not yet well determined. Here, we investigated the role of PLC gamma inhibition in tumor growth and metastasis of human gastric adenocarcinoma using BGC-823 cell line and a nude mouse tumor xenograft model. The results manifested that the depletion of PLC gamma 1 by the transduction with lentivirus-mediated PLC gamma 1 gene short-hairpin RNA (shRNA) vector led to the decrease of tumor growth and metastasis of human gastric adenocarcinoma in vitro and in vivo. Furthermore, the Akt/Bad, Akt/S6, and ERK/Bad signal axes were involved in PLC gamma 1-mediated tumor growth and metastasis of human gastric adenocarcinoma. Therefore, the abrogation of PLC gamma 1 signaling by shRNA could efficaciously suppress human gastric adenocarcinoma tumor growth and metastasis, with important implication for validating PLC gamma 1 as a potential target for human gastric adenocarcinoma.