期刊
ONCOTARGET
卷 7, 期 52, 页码 86266-86279出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.13379
关键词
nonsyndromic cleft palate; nonsyndromic cleft lip with cleft palate; plasma microRNA; miRNA microarray
资金
- National Natural Science Foundation of China [81501672]
- Natural Science Foundation of Jiangsu Province [BK20140083]
- Nanjing Medical Science and Technique Development Foundation [YKK15159]
- Nanjing Science and Technology project [201503047]
Plasma microRNAs (miRNAs) have recently emerged as a new class of regulatory molecules that influence many biological functions. However, the expression profile of plasma microRNAs in nonsyndromic cleft palate (NSCP) or nonsyndromic cleft lip with cleft palate (NSCLP) remains poorly investigated. In this study, we used Agilent human miRNA microarray chips to monitor miRNA levels in three NSCP plasma samples (mixed as the CP group), three NSCLP plasma samples (mixed as the CLP group) and three normal plasma samples (mixed as the Control group). Six selected plasma miRNAs were validated in samples from an additional 16 CP, 33 CLP and 8 healthy children using qRT-PCR. Using Venn diagrams, distinct and overlapping dysregulated miRNAs were identified. Their respective target genes were further assessed using gene ontology and pathway analysis. The results show that distinct or overlapping biological processes and signalling pathways were involved in CP and CLP. Our study showed that the common key gene targets reflected functional relationships to the Notch, Wnt, phosphatidylinositol and Hedgehog signalling pathways. Further studies should examine the mechanism of the potential target genes, which may provide new avenues for future clinical prevention and therapy.
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