期刊
NPG ASIA MATERIALS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/am.2015.148
关键词
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资金
- National Natural Science Foundation of China [11372243, 11522219, 11532009]
- Major International Joint Research Program of China [11120101002]
- International Science and Technology Cooperation Program of China [2013DFG02930]
- Research Fund for the Doctoral Program of Higher Education of China [20130201120071]
- China Postdoctoral Science Foundation [2013M540742]
- China Young 1000-Talent Program
- Program for New Century Excellent Talents in University [NCET-12-0437]
Living cells respond to their mechanical microenvironments during development, healing, tissue remodeling and homeostasis attainment. However, this mechanosensitivity has not yet been established definitively for cells in three-dimensional (3D) culture environments, in part because of challenges associated with providing uniform and consistent 3D environments that can deliver a large range of physiological and pathophysiological strains to cells. Here, we report microscale magnetically actuated, cell-laden hydrogels (mu MACs) for investigating the strain-induced cell response in 3D cultures. mu MACs provide high-throughput arrays of defined 3D cellular microenvironments that undergo reversible, relatively homogeneous deformation following non-contact actuation under external magnetic fields. We present a technique that not only enables the application of these high strains (60%) to cells but also enables simplified microscopy of these specimens under tension. We apply the technique to reveal cellular strain-threshold and saturation behaviors that are substantially different from their 2D analogs, including spreading, proliferation, and differentiation. mu MACs offer insights for mechanotransduction and may also provide a view of how cells respond to the extracellular matrix in a 3D manner.
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