期刊
NEUROSCIENCE BULLETIN
卷 32, 期 4, 页码 331-340出版社
SPRINGER
DOI: 10.1007/s12264-016-0045-6
关键词
Myelination; Neuron-glial interaction; Oligodendrocyte; Lysosome; Rab27b
资金
- National Natural Science Foundation of China [31071251, 81471255]
- National Basic Research Development Program of China [2014CB542202]
- Basic Research Program of the Department of Education, Jiangsu Province, China [14KJA310004]
- Natural Science Research Project of Nantong Science and Technology Bureau, Jiangsu Province, China [HS2013014]
- Natural Science Research Project of Nantong University, Jiangsu Province, China [12Z045, 13Z008]
Myelination by oligodendrocytes in the central nervous system requires coordinated exocytosis and endocytosis of the major myelin protein, proteolipid protein (PLP). Here, we demonstrated that a small GTPase, Rab27b, is involved in PLP trafficking in oligodendrocytes. We showed that PLP co-localized with Rab27b in late endosomes/lysosomes in oligodendrocytes. Short hairpin-mediated knockdown of Rab27b not only reduced lysosomal exocytosis but also greatly diminished the surface expression of PLP in oligodendrocytes. In addition, knockdown of Rab27b reduced the myelin-like membranes induced by co-culture of oligodendrocytes and neurons. Our data suggest that Rab27b is involved in myelin biogenesis by regulating PLP transport from late endosomes/lysosomes to the cell membrane in oligodendrocytes.
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