期刊
NATURE STRUCTURAL & MOLECULAR BIOLOGY
卷 24, 期 1, 页码 61-68出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.3330
关键词
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资金
- NIH [GM061603, GM065402, GM103493]
- Wellcome Trust [094423]
- Science Foundation Ireland [12/IA/1335]
- W.R. Wiley Environmental Molecular Science Laboratory - DOE at Pacific Northwest National Laboratory
- DOE [DE-AC05-76RLO-1830]
- Italian PNRA
- COST action [BM1102]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103493, R37GM065204, R01GM061603] Funding Source: NIH RePORTER
The ribosome can change its reading frame during translation in a process known as programmed ribosomal frameshifting. These rare events are supported by complex mRNA signals. However, we found that the ciliates Euplotes crassus and Euplotes focardii exhibit widespread frameshifting at stop codons. 47 different codons preceding stop signals resulted in either +1 or +2 frameshifts, and +1 frameshifting at AAA was the most frequent. The frameshifts showed unusual plasticity and rapid evolution, and had little influence on translation rates. The proximity of a stop codon to the 3' mRNA end, rather than its occurrence or sequence context, appeared to designate termination. Thus, a 'stop codon' is not a sufficient signal for translation termination, and the default function of stop codons in Euplotes is frameshifting, whereas termination is specific to certain mRNA positions and probably requires additional factors.
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