期刊
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
卷 10, 期 7, 页码 718-727出版社
J INFECTION DEVELOPING COUNTRIES
DOI: 10.3855/jidc.7426
关键词
Escherichia coli; Klebsiella pneumoniae; ESBLs; carbapenemases; Tunisia; Libya
资金
- CNRS
- IHU Mwditerranee Infection
- Ministry of Higher Education and Scientific Research of Tunisia
Introduction: The aim of the study was to investigate the prevalence of extended-spectrum beta-lactamase (ESBL) and carbapenemase production among clinical isolates of Enterobacteriaceae recovered from Tunisian and Libyan hospitals. Methodology: Bacterial isolates were recovered from patients in intensive care units and identified by biochemical tests and MALDI-TOF. Antibiotic susceptibility testing was performed by disk diffusion and the E-test method. ESBL and carbapenemase activities were detected using standard microbiological tests. Antibiotic resistance-encoding genes were screened by PCR and sequencing. Clonal relationships between Klebsiella pneumoniae strains were carried out using multi-locus sequence typing (MLST). Results: A total of 87 isolates were characterized, with 51 and 36, respectively, identified as E. coli and K. pneumoniae. Overall the resistance prevalence was high for aminoglycosides (> 60%), fluoroquinolones (> 80%), and extended-spectrum cephalosporins (> 94%), and was low for imipenem (11.4%). Among this collection, 58 strains (66.6%) were ESBL producers and 10 K. pneumoniae strains (11.4%) were carbapenemase producers. The antibiotic resistance-encoding genes detected were blaC(TX-M-15) (51.7%), bla(TEM-1) (35.6%), several variants of bla(SHV) (21.8%), and bla(OXA-48) (11.4%). The MLST typing of K. pneumoniae isolates revealed the presence of multiple clones and three novel sequence types. Also, close relationships between the OXA-48-producing strains from Tunisia and Libya were demonstrated. Conclusions: This study is the first paper describing the emergence of carbapenemase-and ESBL-producing Enterobacteriaceae, sensitive to colistin, isolated in Tunisia and Libya. Active surveillance and testing for susceptibility to colistin should be implementing because resistance to colistin, mainly in Klebsiella, has been recently reported worldwide.
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