期刊
NATURE COMMUNICATIONS
卷 7, 期 -, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/ncomms12128
关键词
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资金
- EMBL International PhD Program
- EMBO postdoctoral fellowship [LTF 1006-2013]
- Helmholtz Association [VH-NG-1010]
- MRC Career Development Award [MR/L019434/1]
- European Molecular Biology Laboratory
- ERC [ERC-2011-ADG_20110310]
- Medical Research Council [MR/L019434/1] Funding Source: researchfish
- Natural Environment Research Council [NE/I005536/1] Funding Source: researchfish
- MRC [MR/L019434/1, MC_UU_12014/10] Funding Source: UKRI
- NERC [NE/I005536/1] Funding Source: UKRI
The maternal-to-zygotic transition (MZT) is a process that occurs in animal embryos at the earliest developmental stages, during which maternally deposited mRNAs and other molecules are degraded and replaced by products of the zygotic genome. The zygotic genome is not activated immediately upon fertilization, and in the pre-MZT embryo post-transcriptional control by RNA-binding proteins (RBPs) orchestrates the first steps of development. To identify relevant Drosophila RBPs organism-wide, we refined the RNA interactome capture method for comparative analysis of the pre-and post-MZTembryos. We determine 523 proteins as high-confidence RBPs, half of which were not previously reported to bind RNA. Comparison of the RNA interactomes of pre- and post-MZT embryos reveals high dynamicity of the RNA-bound proteome during early development, and suggests active regulation of RNA binding of some RBPs. This resource provides unprecedented insight into the system of RBPs that govern the earliest steps of Drosophila development.
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