期刊
NATURE COMMUNICATIONS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms13025
关键词
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资金
- BBSRC [BB/L01985X/1]
- BBSRC [BB/I004831/1, BB/L01985X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I004831/1, BB/L01985X/1] Funding Source: researchfish
An important goal in synthetic biology is the assembly of biomimetic cell-like structures, which combine multiple biological components in synthetic lipid vesicles. A key limiting assembly step is the incorporation of membrane proteins into the lipid bilayer of the vesicles. Here we present a simple method for delivery of membrane proteins into a lipid bilayer within 5 min. Fusogenic proteoliposomes, containing charged lipids and membrane proteins, fuse with oppositely charged bilayers, with no requirement for detergent or fusion-promoting proteins, and deliver large, fragile membrane protein complexes into the target bilayers. We demonstrate the feasibility of our method by assembling a minimal electron transport chain capable of adenosine triphosphate (ATP) synthesis, combining Escherichia coli F1Fo ATP-synthase and the primary proton pump bo(3)-oxidase, into synthetic lipid vesicles with sizes ranging from 100 nm to similar to 10 mu m. This provides a platform for the combination of multiple sets of membrane protein complexes into cell-like artificial structures.
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