期刊
NATURE COMMUNICATIONS
卷 7, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms11179
关键词
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资金
- European Molecular Biology Organization Long-term Fellowship (EMBO)
- Integrated DNA Technologies (IDT)
- Synthetic Biology Engineering Research Center (SynBERC): NSF [SA5284-11210]
- NSEFF (National Security Science and Engineering Faculty Fellow) [N00014-15-1-0034]
- NSF Expedition in Computing Award [CCF1317291]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1317291] Funding Source: National Science Foundation
The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. Here, we show the ability to express ssDNAs in Escherichia coli (32-205 nt), which can form structures in vivo or be purified for in vitro assembly. Each ssDNA is encoded by a gene that is transcribed into non-coding RNA containing a 30-hairpin (HTBS). HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. Purified ssDNA that is produced in vivo is used to assemble large 1D wires (300 nm) and 2D sheets (5.8 mu m(2)) in vitro. Intracellular assembly is demonstrated using a four-ssDNA crossover nanostructure that recruits split YFP when properly assembled. Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells.
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