期刊
JOURNAL OF FUNCTIONAL FOODS
卷 23, 期 -, 页码 283-293出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2016.02.033
关键词
Persimmon tannin; Cholesterol homeostasis; LXR alpha; ABCA1; CYP7A1
资金
- National Natural Science Foundation of China [31571839]
- Chinese Ministry Program for New Century Excellent Talents in University [NCET-12-0865]
- Special Fund for Agro-scientific Research in the Public Interest [201203047]
Persimmon tannin (proanthocyanidins) has been proven to exert significant hypocholesterolaemic effects in a variety of models. However, the regulatory mechanisms of persimmon tannin on cholesterol metabolism have yet to be fully understood. The present study investigated the regulation of persimmon tannin on the expression of genes critical for cholesterol absorption and cholesterol efflux. Our results indicated that persimmon tannin reduced the uptake of 22-NBD-cholesterol and inhibited the accumulation of cholesterol significantly in HepG2 and Caco-2 cell lines. The mRNA and proteins were quantified using real time PCR and western blot, respectively. The expression of SREBP-2 gene, critical for cholesterol synthesis, and NPC1L1 involved in cholesterol absorption were significantly inhibited by persimmon tannin. Moreover, persimmon tannin showed potential in promoting cholesterol efflux through up-regulating mRNA and/or protein levels of ABCA1, ABCG1, SR-BI, CYP7A1 and ABCGS/G8. However, the regulation effects of persimmon tannin on cholesterol homeostasis in both cell lines were LXR alpha independent. (C) 2016 Elsevier Ltd. All rights reserved.
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