期刊
JOURNAL OF FUNCTIONAL FOODS
卷 21, 期 -, 页码 474-484出版社
ELSEVIER
DOI: 10.1016/j.jff.2015.12.031
关键词
H. erinaceus; Erinacines A; Colorectal cancer cells; ROS; p70S6K; p21
资金
- Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung Memorial Hospital, and Chang Gung University of Science and Technology, Chia-Yi Campus, Taiwan [CLRPG8D0111, CMRPG6E0181, CMRPF6E0011, CMRPF6E0041, CMRPF6E0021, CMRPG8C1262, CMRPG6C0013, CMRPG6C0302, CMRPG6E0191, CMRPF6D0071]
- Ministry of Science and Technology, Taiwan [MOST 103-2622-B-255-001-CC3, MOST 103-2313-B-255-001]
Hericium erinaceus is a well-known edible mushroom with valuable biological properties. Erinacine A is the major active agent of the diterpenoid compounds of the cultured mycelia of H. erinaceus. This agent has multiple physiological activities, including anti-tumourigenic activity. In this study, we investigated the molecular mechanisms by which erinacine A mediated the generation of reactive oxygen species (ROS), and we performed cell cycle arrest to clarify molecular changes in colorectal cancer cells (CRC). Treatment of DLD-1 cells with erinacine A resulted in the phosphorylation of c-Jun N-terminal kinase (JNK) and p70S6K, the activation of p50 and p-IKB-beta protein levels, the downregulation of cell-cycle-related proteins (cyclin A, cdk2, cyclin E, cdk4, and cyclin D1), and the induction of p21. Furthermore, treatment with the N-acetyl cysteine (NAC) and mTOR inhibitor (rapamycin) abolished erinacine A-induced cell cycle G1 arrest and reversed the association of CDK2 with Cyclin E and CDK4 with Cyclin D1. Therefore, when DLD-1 cells were grown as xenografts in nude mice, treatment with erinacine A induced a significant dose-dependent decrease in tumour growth. Histochemical and immunohistochemical analysis revealed that erinacine A treatment significantly reduced the number of mitotic cells. These results suggest that erinacine
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