期刊
ONCOTARGETS AND THERAPY
卷 9, 期 -, 页码 4473-4481出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/OTT.S100593
关键词
TNFAIP8; gastric cancer; shRNA; anti-DR5ScFv; metastasis; apoptosis
资金
- National Natural Science Foundation of China [81272720]
- Fujian province [3402Z20100002]
- Medical innovation project Fujian province [2014-CXB-43]
- Public Projects of Fujian Province [2016R1034-3]
- key projects for technology plan of Xiamen city
Background: TNFAIP8, also known as TIPE, is a suppressor of apoptosis. High expression of both TIPE mRNA and protein has been detected in various cancer cell lines and clinical specimens compared to healthy tissues. Many reports have shown that there is a strong correlation between TIPE overexpression and cancer progression and poor prognosis in human solid cancers. Methods: To illustrate the functional and clinical significance of TIPE in gastric cancer, we used reverse transcription polymerase chain reaction, quantitative real-time polymerase chain reaction, and immunohistochemistry to measure TIPE expression in clinical gastric specimens. Then, TIPE expression was knocked down by using shRNA and anti-DR5ScFv, to examine different expressions of TIPE in BGC823 cell lines, while cell proliferation and apoptosis were induced. Results: We found that there was a strong correlation between TIPE expression and TNM stage (P=0.044), tumor depth (P=0.016), lymph node metastasis (P=0.026), and distant metastasis (P=0.045). No significant correlation was found between TIPE expression with the patients' age (P=0.062) or sex (P=0.459). Anti-DR5ScFv induced TIPE depletion both in vitro and in vivo and resulted in apoptosis and suppression of proliferation. Conclusion: Our results suggested that TIPE expression was associated with gastric cancer progression, and most importantly, suppressing TIPE expression might be an effective therapeutic strategy.
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