期刊
BIOANALYSIS
卷 8, 期 8, 页码 847-856出版社
FUTURE SCI LTD
DOI: 10.4155/bio.16.24
关键词
acid hydrolysis; enzyme digestion; immunocapture; LC-MS/MS; protein quantitation; regulated bioanalysis
资金
- BMS
LC-MS/MS has been investigated to quantify protein therapeutics in biological matrices. The protein therapeutics is digested by an enzyme to generate surrogate peptide(s) before LC-MS/MS analysis. One challenge is isolating protein therapeutics in the presence of large number of endogenous proteins in biological matrices. Immunocapture, in which a capture agent is used to preferentially bind the protein therapeutics over other proteins, is gaining traction. The protein therapeutics is eluted for digestion and LC-MS/MS analysis. One area of tremendous potential for immunocapture-LC-MS/MS is to obtain quantitative data where ligand-binding assay alone is not sufficient, for example, quantitation of antidrug antibody complexes. Herein, we present an overview of recent advance in enzyme digestion and immunocapture applicable to protein quantitation.
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