期刊
BIOANALYSIS
卷 8, 期 15, 页码 1579-1595出版社
FUTURE SCI LTD
DOI: 10.4155/bio-2016-0012
关键词
catabolites; high-resolution accurate mass MS; immunoaffinity enrichment; orbitrap; oxyntomodulin; quantification; validation
Background: A thorough understanding of the biological role of oxyntomodulin (OXM) has been limited by the availability of sensitive and specific analytical tools for reliable in vivo characterization. Here, we utilized immunoaffinity capture coupled with high- resolution accurate mass LC- MS detection to quantify OXM and its primary catabolites. Results: Quantification of intact OXM 1-37 in human and rat plasma occurred in pre- and post-prandial samples. Profiles for the major catabolites were observed allowing kinetic differences to be assessed between species. Conclusion: A validated assay in human and rat plasma was obtained for OXM 1-37 and its catabolites, 3-37 and 4-37. The value of full scan high- resolution accurate mass detection without selected reaction monitoring for low- abundance peptide quantification was also demonstrated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据