期刊
BIOANALYSIS
卷 8, 期 2, 页码 143-155出版社
FUTURE SCI LTD
DOI: 10.4155/bio.15.234
关键词
dPCR; hybridization; LC-fluorescence; LC-HRAM; LC-MS; MS; oligonucleotide; qPCR; siRNA
Technical advances and demands for high-throughput accurate quantification of oligonucleotide therapeutics and biomarkers in pharmaceutical research and clinical diagnosis have aided evolution in quantitative bioanalysis of oligonucleotides. Many bioanalytical methods are available for absolute quantification of oligonucleotides in biological matrices. They can be broadly classified into two categories: hybridization-based assays commonly used by molecular biologists and chromatographic assays routinely used by chemists. Each category has its own advantages and disadvantages for specific applications. This review summarizes the mechanisms and applications of some of the current most commonly used techniques in each category.
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