期刊
CHEMICAL SCIENCE
卷 7, 期 7, 页码 4245-4250出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6sc00055j
关键词
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资金
- Ministry of Defence [R279-000-340-232]
- SMART [R279-000-378-592]
- Ministry of Education [R279-000-391-112]
- Singapore NRF Investigatorship [R279-000-444-281]
- Institute of Materials Research and Engineering of Singapore [IMRE/14-8P1110]
The accurate detection of biological substances is highly desirable to study various biological processes and evaluate disease progression. Herein, we report a self-validated fluorescent probe which is composed of a coumarin fluorophore as the energy donor and a fluorogen with aggregation-induced emission characteristics (AIEgen) as the energy quencher linked through a caspase-3 specific peptide substrate. Unlike the traditionally widely studied fluorescence resonance energy transfer (FRET) probes, our new generation of FRET probe is non-fluorescent itself due to the energy transfer as well as the dissipation of the acceptor energy through the free molecular motion of AIEgen. Upon interaction with caspase-3, the probe displays strong green and red fluorescent signals synchronously due to the separation of the donor-quencher and aggregation of the released AIEgen. The fluorescence turn-on with dual signal amplification allows real-time and self-validated enzyme detection with a high signal-to-background ratio, providing a good opportunity to accurately monitor various biological processes in a real-time manner.
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