期刊
CHEMICAL SCIENCE
卷 7, 期 3, 页码 1891-1895出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5sc03404c
关键词
-
资金
- MOST [2014CB542500, 2013CB932800, 2014AA020527]
- NSFC [81361120384, 21532004, 31270913, 81422020]
- Specialized Research Fund for the Doctoral Program of Higher Education [20120002130004]
We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen-antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K96NPE, was totally inert to hen egg lysozyme (HEL)-specific B cells and could only restore its antigenicity upon photoactivation. Combined with real time live cell imaging, the utility of HEL-K96NPE was demonstrated as a proof of concept to quantify B cell synapse formation and calcium influx responses at the single cell level.
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