Effect of lentivirus-mediated integrin α V β 3-shRNA on tumor growth of mice with lung cancer xenografts

Objective: To study the effect of lentivirus-mediated integrin alpha V beta 3-shRNA on tumor growth of mice with lung cancer xenograft. Methods: Lung cancer tissue, paracancer tissue and normal tissue were collected and integrin alpha V beta 3 expression was detected; BALB/c nude mice were selected, divided into integrin alpha V beta 3 knockdown group (KD group) and negative control group (NC group), and inoculated with cells stably infected by integrin alpha V beta 3-shRNA lentivirus and cells stably infected by negative control-shRNA lentivirus respectively, the growth of tumor tissue was continuously observed, and the number of apoptosis cells as well as the expression of angiogenesis, apoptosis and invasion genes in tumor tissue were detected. Results: mRNA content and protein content of integrin alpha V beta 3 in lung cancer tissue were significantly higher than those in paracancer tissue and normal tissue; increasing trend of tumor tissue volume of KD group was weaker than that of NC group, and tumor volume at various points in time of KD group was tower than that of NC group; mRNA contents and protein contents of VEGF, FGF, EGF, Bcl-2, MMP-9, MMP-12 and MMP-13 in tumor tissue of KD group were lower than those of NC group, and apoptosis index as well as mRNA content and protein content of Box were higher than those of NC group. Conclusions: The expression of integrin alpha V beta 3 increases in lung cancer tissue, and lentivirus-mediated integtin alpha V beta 3-shRNA can inhibit tumor growth of mice with lung cancer xenografts.