期刊
YONSEI MEDICAL JOURNAL
卷 57, 期 3, 页码 790-794出版社
YONSEI UNIV COLL MEDICINE
DOI: 10.3349/ymj.2016.57.3.790
关键词
AAV-DJ; large scale production; liver-specific expression
资金
- National Institutes of Health [HL020948]
- New Faculty Grant from Inha University [51362-01]
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expression of a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simple large-scale preparation method that can generate 3x10(13) viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
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