期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 33, 期 1, 页码 -出版社
SPRINGER
DOI: 10.1007/s11274-016-2156-7
关键词
Acarbose; Actinomyces; ARTP; Mutation breeding; Cell wall components
资金
- National High Technology Research and Development program of china (863) [2007AA10Z362]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Atmospheric and room temperature plasma (ARTP) was first employed to generate mutants of Actinomyces JN537 for improving acarbose production. To obtain higher acarbose producing strains, the method of screening the strains for susceptibility to penicillin was used after treatment with ARTP. The rationale for the strategy was that mutants showing penicillin susceptibility were likely to be high acarbose producers, as their ability to synthesize cell walls was weak which might enhance metabolic flux to the pathway of acarbose biosynthesis. Acarbose yield of the mutant strain M37 increased by 62.5 % than that of the original strain. The contents of monosaccharides and amino acids of the cell wall of M37 were lower than that of the original strain. The acarbose production ability in mutant strain remained relatively stable after 10 generations. This work provides a promising strategy for obtaining high acarbose-yield strains by combination of ARTP mutation method and efficient screening technique.
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