期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 32, 期 4, 页码 -出版社
SPRINGER
DOI: 10.1007/s11274-016-2022-7
关键词
Butanol; Cassava; Clostridium acetobutylicum; N-methyl-N-nitro-N-nitrosoguanidine mutation; Genome shuffling
资金
- Guangxi Science and Technology Lab Center [LGZX201006]
- National Natural Science Foundation of China [31560027]
- Bagui Scholars Distinguished Professor Special Project
To obtain strains exhibiting high levels of solvent tolerance and butanol production, wild type strains of Clostridium acetobutylicum butanol-producing strain GX01 and Lactobacillus mucosae butanol-tolerant strain M26 were subjected to mutagenesis combining N-methyl-N-nitro-N-nitrosoguanidine induction with genome shuffling. After four successive rounds of genome shuffling, the C. acetobutylicum shuffled strain GS4-3 showing greater levels of fermentation performances (such as secreting a higher level of amylase, improving the thermal stability, and possessing greater environmental robustness) compared to the wild type strains was isolated. As a result, after optimization of culture conditions, mutant GS4-3 produced 32.6 g/L of total solvent, 20.1 g/L of butanol production, and 0.35 g/L/h of butanol productivity, which were, respectively, increased by 23.5, 23.3, and 40.0 % than the wild-type strain GX01, in a 10 L bioreactor. The enhanced production of butanol and tolerance of solvent of mutant associated with GS4-3 make it promising for acetone/butanol/ethanol fermentation from cassava (Manihot esculenta).
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