期刊
WATER SCIENCE AND TECHNOLOGY
卷 74, 期 4, 页码 824-835出版社
IWA PUBLISHING
DOI: 10.2166/wst.2016.170
关键词
anaerobic prokaryote community; clay residuals; co-digestion; rice straw; swine manure
资金
- German Academic Exchange Service DAAD [A/12/94350]
- German Federal Ministry of Food, Agriculture and Consumer Protection BMELV [22010711, 22016308]
The aim of this study was to analyze the effect of the addition of rice straw and clay residuals on the prokaryote methane-producing community structure in a semi-continuously stirred tank reactor fed with swine manure. Molecular techniques, including terminal restriction fragment length polymorphism and a comparative nucleotide sequence analyses of the prokaryotic 16S rRNA genes, were performed. The results showed a positive effect of clay addition on methane yield during the co-digestion of swine manure and rice straw. At the digestion of swine manure, the bacterial phylum Firmicutes and the archaeal family Methanosarcinaceae, particularly Methanosarcina species, were predominant. During the co-digestion of swine manure and rice straw the microbial community changed, and with the addition of clay residual, the phylum Bacteroidetes predominated. The new nutritional conditions resulted in a shift in the archaeal family Methanosarcinaceae community as acetoclastic Methanosaeta species became dominant.
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