期刊
WATER RESEARCH
卷 102, 期 -, 页码 305-312出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2016.06.052
关键词
Murine norovirus; Underwater arc discharge; Capsid protein
资金
- Korea Research Food Institute (KFRI) [E0142103]
- Ministry of Science, ICT and Future Planning of the Republic of Korea
In this study, we investigated the inactivation effects on murine norovirus (MNV-1) with/without purification in water using a submerged plasma reactor of arc discharge (underwater arc), which produced a shockwave, UV light, reactive oxygen species and reactive nitrogen species. Underwater arc treatments of 3 and 6 Hz at 12 kV resulted in 2.6- and 4.2-log reductions in the virus titer of non-purified MNV-1 after 1 min of treatment, respectively. The reduction of purified MNV-1 was higher than that of non purified MNV-1 after underwater arc treatment for all applied conditions (12 or 15 kV and 3 or 6 Hz). One of the viral capsid proteins (VP1) was not detectable after underwater arc treatment, when its integrity was assessed by western blot analysis. Transmission electron microscopy analysis also revealed that MNV-1 particles were completely dissembled by the treatment. This study demonstrates that underwater arc treatment, which was capable of disintegrating the MNV-1 virion structure and the viral capsid protein, can be an effective disinfection process for the inactivation of water-borne noroviruses. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据