期刊
WATER RESEARCH
卷 102, 期 -, 页码 524-532出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2016.07.002
关键词
Fermentative hydrogen production; Waste activated sludge; Homoacetoogenesis; Alkaline fermentation; Mesophilic; Thermophilic
资金
- Science and Technology Commission of Shanghai Municipality [14YF1400400]
- National Natural Science Foundation of China [51408133]
- SRF for ROCS, SEM
The present study compared the mesophilic and thermophilic alkaline fermentation of waste activated sludge (WAS) for hydrogen production with focus on homoacetogenesis, which mediated the consumption of H-2 and CO2 for acetate production. Batch experiments showed that hydrogen yield of WAS increased from 19.2 mL H-2/gVSS at 37 degrees C and pH 10-80.1 mL H-2/gVSS at 55 degrees C and pH 10. However, the production of volatile fatty acids (mainly acetate) was higher at 37 degrees C and pH 10 by comparison with 55 degrees C and pH 10. Hydrogen consumption due to homoacetogenesis was observed at 37 degrees C and pH 10 but not 55 degrees C and pH 10. Higher expression levels of genes relating with homoacetogenesis and lower expression levels of genes relating with hydrogen production were found at 37 degrees C and pH 10 compared to 55 degrees C and pH 10. The continuous experiment demonstrated the steady-state hydrogen yield of WAS was comparable to that obtained from batch experiments at 55 degrees C and pH 10, and homoacetogenesis was still inhibited. However, the steady-state hydrogen yield of WAS (6.5 mL H-2/gVSS) was much lower than that (19.2 mL H-2/gVSS) obtained from batch experiments at 37 degrees C and pH 10 due to the gradual enrichment of homoacetogens as demonstrated by qPCR analysis. The high-throughput sequencing analysis of 16S rRNA genes showed that the abundance of genus Clostridium, containing several homoacetogens, was 5 times higher at 37 degrees C and pH 10 than 55 degrees C and pH 10. (C) 2016 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据