期刊
WATER RESEARCH
卷 102, 期 -, 页码 362-373出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2016.06.038
关键词
Syntrophic acetate oxidation; Mathematical model; Stable carbon isotopes; Anaerobic biofilm; Carbon isotope fractionation
资金
- German Research Foundation DFG [LU 1167/5-1]
In order to accurately describe the carbon flow in anaerobic digestion processes, this work investigates the acetate degradation pathways through the use of stable carbon isotope analysis and a mathematical model. Batch assays using labeled C-13 acetate were employed to distinguish the acetate consumption through methanogenic Archaea and acetate-oxidizing Bacteria. Suspended and sessile biomass, with over 400 days of retention time, from a mesophilic (36.5 degrees C) upflow anaerobic filter was used as inocula in these assays. A three-process model for acetoclastic methanogenesis and syntrophic acetate oxidation (SAO) was developed to allow for a precise quantification of the SAO contribution. The model distinguishes carbon atoms in light and heavy isotopes, C-12 and C-13, respectively, which permitted the simulation of the isotope ratios variation in addition to gas production, gas composition and acetate concentrations. The model indicated oxidized fractions of acetate between 7 and 18%. Due to the low free ammonia inhibition potential for the acetoclastic methanogens in these assays these findings point to the biomass retention times as a driven factor for the SAO pathway. The isotope-based kinetic model developed here also describes the delta C-13 variations in unlabeled assays accurately and has the potential to determine biological C-13 fractionation factors. (C) 2016 Elsevier Ltd. All rights reserved.
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