期刊
VOX SANGUINIS
卷 111, 期 1, 页码 22-32出版社
WILEY-BLACKWELL
DOI: 10.1111/vox.12390
关键词
blood component; extracellular vesicles; manufacture; microparticles; mitochondrial DNA; process; red blood cells; storage
类别
资金
- Blood Systems Research Institute
- National Institutes of Health, National Heart, Lung, and Blood Institute [HL095470]
- Canadian Blood Services - federal (Health Canada), provincial and territorial Ministries of Health
Background and Objectives Damage-associated molecular patterns (DAMPs) are found in transfusion products, but their potential impacts are not fully understood. We examined the influence of manufacturing method on levels of mitochondrial (mt) DNA and extracellular vesicle (EV) DAMPs in red cell concentrates (RCCs). Materials and Methods Eighty-seven RCCs were prepared using nine different methods (6-15 units/method), including three apheresis, five whole blood (WB)-derived leucoreduced (LR) and one WB-derived non-LR method. On storage days 5 and 42, levels of mtDNA (by PCR) and number and cell of origin of EVs (by flow cytometry) were assessed in RCC supernatants. Results There was a 100-fold difference in mtDNA levels among methods, with highest levels in non-LR, followed by MCS+ and Trima apheresis RCCs. There was a 10-fold difference in EV levels among methods. RBC-derived CD235a+ EVs were found in fresh RCCs and increased in most during storage. Platelet-derived CD41a+ EVs were highest in non-LR and Trima RCCs and did not change during storage. WBC-derived EVs were low in most RCCs; CD14+ EVs increased in several RCCs during storage. Conclusion DAMPs in RCCs vary by manufacturing method. MtDNA and EV could be informative quality markers that may be relevant to RCC immunomodulatory potential.
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