期刊
VISUAL NEUROSCIENCE
卷 33, 期 -, 页码 -出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0952523815000310
关键词
Retinal pigment epithelium; Cell nucleus; Bi-nucleate; Multi-nucleate
资金
- German Research Foundation DFG (Bonn, Germany) [AC265/1-1, AC265/2-1]
- National Institutes of Health (Bethesda, Maryland, USA) [R01 EY06109, R01 EY015520, EY019065]
- Research to Prevent Blindness
- EyeSight Foundation of Alabama
- NATIONAL EYE INSTITUTE [R01EY015520, R01EY019065, R01EY006109] Funding Source: NIH RePORTER
Background : The human retinal pigment epithelium (RPE) is reportedly 3% bi-nucleated. The importance to human vision of multi-nucleated (MN)-RPE cells could be clarified with more data about their distribution in central retina. Methods : Nineteen human RPE-flatmounts (9 <= 51 years, 10 > 80 years) were imaged at 12 locations: 3 eccentricities (fovea, perifovea, near periphery) in 4 quadrants (superior, inferior, temporal, nasal). Image stacks of lipofuscin-attributable autofluorescence and phalloidin labeled F-actin cytoskeleton were obtained using a confocal fluorescence microscope. Nuclei were devoid of autofluorescence and were marked using morphometric software. Cell areas were approximated by Voronoi regions. Mean number of nuclei per cell among eccentricity/quadrant groups and by age were compared using Poisson and binominal regression models. Results : A total of 11,403 RPE cells at 200 locations were analyzed: 94.66% mono-, 5.31% bi-, 0.02% tri-nucleate, and 0.01% with 5 nuclei. Age had no effect on number of nuclei. There were significant regional differences: highest frequencies of MN-cells were found at the perifovea (9.9%) and near periphery (6.8%). The fovea lacked MN-cells almost entirely. The nasal quadrant had significantly more MN-cells compared to other quadrants, at all eccentricities. Conclusion : This study demonstrates MN-RPE cells in human macula. MN-cells may arise due to endoreplication, cell fusion, or incomplete cell division. The topography of MN-RPE cells follows the topography of photoreceptors; with near-absence at the fovea (cones only) and high frequency at perifovea (highest rod density). This distribution might reflect specific requirements of retinal metabolism or other mechanisms addressable in further studies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据