期刊
VIROLOGY
卷 497, 期 -, 页码 185-197出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.07.022
关键词
Coronavirus; Spike protein; Microtubule; Assembly; Viral infectivity; Intracellular transport; Interaction; Incorporation; TGEV
类别
资金
- German Research Foundation (DFG) [SCHW 1408/1.1]
- Bundesministerium fuer Bildung and Forschung of the German Government (Zoonosis Network, Consortium on ecology and pathogenesis of SARS) [01KI1005A, 01KI1005F]
- Emmy Noether Programme from the DFG
- Ministry for Science and Culture of Lower Saxony
Coronavirus spike proteins mediate host-cell-attachment and virus entry. Virus replication takes place within the host cell cytosol, whereas assembly and budding occur at the endoplasmic reticulum-Golgi intermediate compartment. In this study we demonstrated that the last 39 amino acid stretches of Alphacoronavirus spike cytoplasmic domains of the human coronavirus 229E, NL63, and the porcine transmissible gastroenteritis virus TGEV interact with tubulin alpha and beta chains. In addition, a partial co-localization of TGEV spike proteins with authentic host cell beta-tubulin was observed. Furthermore, drug-induced microtubule depolymerization led to changes in spike protein distribution, a reduction in the release of infectious virus particles and less amount of spike protein incorporated into virions. These data demonstrate that interaction of Alphacoronavirus spike proteins with tubulin supports S protein transport and incorporation into virus particles. (C) 2016 Elsevier Inc. All rights reserved.
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