期刊
VIROLOGY
卷 498, 期 -, 页码 82-93出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.08.011
关键词
Porcine circovirus type 2 (PCV2); Porcine circovirus-associated disease (PCVAD); DNA shuffling; Capsid; Vaccine
类别
资金
- Zoetis Inc, Kalamazoo, MI (Virginia Tech internal grant ) [457354]
- BBSRC [BBS/E/D/20241865, BBS/E/D/20241866, BBS/E/D/20241864] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/D/20241866, BBS/E/D/20241864, BBS/E/D/20241865] Funding Source: researchfish
Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated disease (PCVAD). Available commercial vaccines all target PCV2a subtype, although the circulating predominant subtype worldwide is PCV2b, and the emerging PCV2d subtype is also increasingly associated with PCVAD. Here we molecularly bred genetically-divergent strains representing PCV2a, PCV2b, PCV2c, PCV2d, and divergent PCV2a subtypes by DNA-shuffling of the capsid genes to produce a chimeric virus representing PCV2 global genetic diversity. When placed in the PCV2a backbone, one chimeric virus (PCV2-3c114) induced higher neutralizing antibody titers against different PCV2 subtypes. Subsequently, a candidate vaccine (PCV1-3cl14) was produced by cloning the shuffled 3cl14 capsid into the backbone of the non-pathogenic PCV1. A vaccine efficacy study revealed that chimeric virus PCV1-3cl14 induces protective immunity against challenge with PCV2b or PCV2d in pigs. The chimeric PCV1-3cl14 virus is a strong candidate for a novel vaccine in pigs infected with variable PCV2 strains. (C) 2016 Elsevier Inc. All rights reserved.
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