期刊
VIROLOGY
卷 488, 期 -, 页码 129-136出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.11.008
关键词
Closteroviridae; Crinivirus; Tomato chlorosis virus; RNA silencing suppressor; RNA binding protein
类别
资金
- AECID (Spain) fellowship
- CSIC (Spain) [I3P-PC2004L]
- Ministerio de Economia y Competitividad, Spain [AGL2010-22287-C02-01/AGL, AGL2013-48913-C2-1-R]
- Consejeria de Economia, Innovacion y Ciencia, Junta de Andalucia, Spain [AGR-214]
Viruses encode silencing suppressor proteins to counteract RNA silencing. Because dsRNA plays a key role in silencing, a general silencing suppressor strategy is dsRNA binding. The p22 suppressor of the plant virus Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) has been described as having one of the longest lasting local suppressor activities. However, the mechanism of action of p22 has not been characterized. Here, we show that ToCV p22 binds long dsRNAs in vitro, thus interfering with their processing into small RNAs (sRNAs) by an RNase III-type Dicer homolog enzyme. Additionally, we have studied whether a putative zinc finger motif found in p22 has a role in dsRNA binding and suppressor function. The efficient ability of p22 to suppress RNA silencing, triggered by hairpin transcripts transiently expressed in planta, supports the relationship between its ability to bind dsRNA in vitro and its ability to inhibit RNA silencing in vivo. (C) 2015 Elsevier Inc. All rights reserved.
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