期刊
VIROLOGY
卷 489, 期 -, 页码 202-211出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2015.12.013
关键词
FgV1; Fusarium graminearum; FgHex1; Viral RNA accumulation; EMSA; vRNA-host protein interaction
类别
资金
- Center for Fungal Pathogenesis - Ministry of Science, ICT and Future Planning [20110000959]
- Next-Generation BioGreen 21 Program - Rural Development Administration [PJ01101401]
- Vegetable Breeding Research Center through the Agriculture Research Center program from the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [710001-07-05]
- Brain Korea 21 Plus Program
The HEX1 gene of Fusarium graminearum was previously reported to be required for the efficient accumulation of Fusarium graminearum virus 1 (FgV1) RNA in its host. To investigate the molecular mechanism underlying the production of FgHEX1 and the replication of FgV1 viral RNA, we conducted electrophoretic mobility shift assays (EMSA) with recombinant FgHex1 protein and RNA sequences derived from various regions of FgV1 genomic RNA. These analyses demonstrated that FgHex1 and both the 5'- and 3'-untranslated regions of plus-strand FgV1 RNA formed complexes. To determine whether FgHex1 protein affects FgV1 replication, we quantified accumulation viral RNAs in protoplasts and showed that both (+)- and (-)-strands of FgV1 RNAs were increased in the over-expression mutant and decreased in the deletion mutant. These results indicate that the FgHex1 functions in the synthesis of both strands of FgV1 RNA and therefore in FgV1 replication probably by specifically binding to the FgV1 genomic RNA. (C) 2016 Published by Elsevier Inc.
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