期刊
VETERINARY MICROBIOLOGY
卷 188, 期 -, 页码 1-11出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2016.03.008
关键词
Swine; Microbiome; Porcine reproductive and respiratory syndrome virus; Porcine circovirus type 2; Clinical outcome; Virus load
资金
- USDA NIFA Award [2013-68004-20362]
- State of Kansas National Bio and Agro-Defense Facility Fund
- Lawrence Livermore National Laboratory Derived Research and Development effort [14ERD081]
- NIFA [688165, 2013-68004-20362] Funding Source: Federal RePORTER
On a world-wide basis, co-infections involving porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are common and contribute to a range of polymicrobial disease syndromes in swine. Both viruses compromise host defenses, resulting in increased susceptibility to infections by primary and secondary pathogens that can affect growth performance as well as increased morbidity and mortality. An experimental population of 95 pigs was co-infected with PRRSV and PCV2. At 70 days post-infection (dpi), 20 representative pigs were selected as having the best or worst clinical outcome based on average daily gain (ADG) and the presence of clinical disease. Worst clinical outcome pigs had prolonged and greater levels of viremia as measured by qPCR. Serum, lung and fecal samples collected at 70 dpi were analyzed using a comprehensive DNA microarray technology, the Lawrence Livermore Microbial Detection Array, to detect over 8000 microbes. Bacterial species, such as Bacillus cereus, were detected at a higher rate in the serum of worst performing pigs. At the level of the fecal microbiome, the overall microbial diversity was lower in the worst clinical outcome group. The results reinforce the importance of pathogen load in determining clinical outcome and suggest an important role of microbial diversity as a contributing factor in disease. (C) 2016 Elsevier B.V. All rights reserved.
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