期刊
VASCULAR PHARMACOLOGY
卷 87, 期 -, 页码 180-189出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.vph.2016.09.008
关键词
17-Phenyl-trinor-prostaglandin E-2; EP receptors; Endothelial barrier function; Vascular hyperpermeability; Platelet aggregation; Pulmonary inflammation
资金
- FWF [DK-MOLIN - W1241, P22521, P25531, P26185]
- DOC Fellowship of the Austrian Academy of Science at the Institute of Experimental and Clinical Pharmacology [24409]
- Austrian Science Fund (FWF) [P26185, W1241, P25531] Funding Source: Austrian Science Fund (FWF)
Endothelial dysfunction is a hallmark of inflammatory conditions. We recently demonstrated that prostaglandin (PG)E-2 enhances the resistance of pulmonary endothelium in vitro and counteracts lipopolysaccharide (LPS)-induced pulmonary inflammation in vivo via EP4 receptors. The aim of this study was to investigate the role of the EP1/EP3 receptor agonist 17-phenyl-trinor-(pt)-PGE(2) on acute lung inflammation in a mouse model. In LPS-induced pulmonary inflammation in mice, 17-pt-PGE(2) reduced neutrophil infiltration and inhibited vascular leakage. These effects were unaltered by an EP1 antagonist, but reversed by EP4 receptor antagonists. 17-pt-PGE2 increased the resistance of pulmonary microvascular endothelial cells and prevented thrombin-induced disruption of endothelial junctions. Again, these effects were not mediated via EP1 or EP3 but through activation of the EP4 receptor, as demonstrated by the lack of effect of more selective EP1 and EP3 receptor agonists, prevention of these effects by EP4 antagonists and EP4 receptor knock-down by siRNA. In contrast, the aggregation enhancing effect of 17-pt-PGE(2) in human platelets was mediated via EP3 receptors. Our results demonstrate that 17-pt-PGE(2) enhances the endothelial barrier in vitro on pulmonary microvascular endothelial cells, and accordingly ameliorates the recruitment of neutrophils, via EP4 receptors in vivo. This suggests a beneficial effect of 17-pt-PGE(2) on pulmonary inflammatory diseases. (C) 2016 The Authors. Published by Elsevier Inc.
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