4.5 Article

The intranasal vaccination of pregnant dams with Intimin and EspB confers protection in neonatal mice from Escherichia coli (EHEC) O157:H7 infection

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VACCINE
卷 34, 期 25, 页码 2793-2797

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ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2016.04.056

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  1. PICT grant from FONCYT (Argentina) [32687]

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Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of gamma-intimin (gamma-Int C-280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both gamma-Int C-280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against gamma-IntC(280) and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with gamma-Int C-280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. (C) 2016 Elsevier Ltd. All rights reserved.

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