期刊
VACCINE
卷 34, 期 11, 页码 1343-1349出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2016.01.053
关键词
Influenza virus; Cold-adapted live vaccine; X-31; Phenotype; Nucleoprotein
资金
- Vaccine Translational Research Center - Ministry of Health and Welfare of Korean government [HI13C0826]
- National Research Foundation of Korea (NRF) [NRF2015R1D1A4A01016640, NRF2014M3A9E4064743]
Cold-adapted live attenuated influenza vaccines (CAIVs) have been considered as a safe prophylactic measure to prevent influenza virus infections. The safety of a CAIV depends largely on genetic markers that confer specific attenuation phenotypes. Previous studies with other CAIVs reported that polymerase genes were primarily responsible for the attenuation. Here, we analyzed the genetic mutations and their phenotypic contribution in the X-31 ca strain, a recently developed alternative CAIV donor strain. During the cold-adaptation of its parental X-31 virus, various numbers of sequence changes were accumulated in all six internal genes. Phenotypic analysis with single-gene and multiple-gene reassortant viruses suggests that NP gene makes the largest contribution to the cold-adapted (ca) and temperature-sensitive (ts) characters, while the remaining other internal genes also impart attenuation characters with varying degrees. A balanced contribution of all internal genes to the attenuation suggests that X-31 ca could serve as an ideal master donor strain for CAIVs preventing influenza epidemics and pandemics. (C) 2016 Elsevier Ltd. All rights reserved.
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