期刊
UROLOGIA INTERNATIONALIS
卷 98, 期 1, 页码 102-110出版社
KARGER
DOI: 10.1159/000445252
关键词
E2F transcription factor 5; Prostate cancer; miR-154-5p
资金
- National Natural Science Foundation of China [81171963, 81102089, 81201998, 81372757]
Background: MicroRNAs (miRNAs) are a class of small non coding RNAs (18-25 nucleotides) which post-transcriptionally regulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. This study aimed to determine the function of miR-154-5p in prostate cancer (PCa) cells and identify the novel molecular targets regulated by miR-154-5p. Materials and Methods: The effects offorced miR-154-5p expression or E2F transcription factor 5 (E2F5) knockdown on PCa cells were evaluated by cell proliferation, flow cytonnetry, cell migration and invasion assays as well as by Western blot analysis. Dual-luciferase reporter assay was performed to verify the precise target of miR-154-5p. Results: The forced expression of miR-154-5p or E2F5 knockdown significantly restrained cell growth, as well as the migratory and invasive capabilities. Such expression also induced G1 cell cycle arrest of PCa cells in vitro. Hence, E2F5 is a direct target gene of miR-154-5p. Conclusions: miR-154-5p may play an important role as an inhibitor of proliferation, migration and invasion of PCa by targeting E2F5 in PCa cell lines. (C) 2016 S. Karger AG, Basel
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