期刊
ULTRASONICS SONOCHEMISTRY
卷 28, 期 -, 页码 54-61出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2015.06.032
关键词
Ultrasound; Liposome; Perfluorocarbon; HVJ envelope; Avidin; Cancer cells
资金
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [19300182]
- Grants-in-Aid for Scientific Research [15H04193] Funding Source: KAKEN
This study investigated the targeting and ultrasound-triggered injury of cancer cells using anticancer drug-free liposomes that contained an emulsion of perfluoropentane (ePFC5) and were co-modified with avidin as a targeting ligand for cancer cells and the hemagglutinating virus of Japan (HVJ) envelope to promote liposome fusion with the cells. These liposomes are designated as ePFC5-loaded avidin/HVJ liposomes. ePFC5-loaded liposomes were sensitized to ultrasound irradiation. Liposomes modified with avidin alone (avidin liposomes) showed binding to MCF-7 human breast cancer cells, and liposomes modified with HVJ envelope alone (HVJ liposomes) were found to fuse with MCF-7 cells. The irradiation of MCF-7 cells with 1 MHz ultrasound (30 s, 1.2 W/cm(2), duty ratio 30%) combined with ePFC5-loaded avidin/HVJ liposomes resulted in a decrease in cell viability at 1 h after irradiation to 43% of that of controls without ultrasound irradiation or liposomes. The cell viability was lower than that of cells treated with ultrasound irradiation with ePFC5-loaded avidin liposomes or ePFC5-loaded HVJ liposomes. This indicates that co-modification of liposome with avidin and HVJ envelope could enhance ultrasound-induced cell injury in the presence of ePFC5-loaded liposomes. (C) 2015 Elsevier B.V. All rights reserved.
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