期刊
TUMOR BIOLOGY
卷 37, 期 8, 页码 10085-10096出版社
SAGE PUBLICATIONS LTD
DOI: 10.1007/s13277-016-4803-x
关键词
Hepatocellular carcinoma; Alpha-fetoprotein; Single-chain variable fragment; Apoptosis; Cell cycle arrest
类别
资金
- Chinese Universities Scientific Fund [ZJ13083]
- Fundamental Research Funds for the Central Universities [YD2014SK0002]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
Human hepatocellular carcinoma (HCC) has a high rate of tumor recurrence and metastasis, resulting in shortened survival time. The function of alpha-fetoprotein (AFP) as a regulatory factor in the growth of HCC cells has been well defined. The aim of this study was to investigate the use of a novel AFP-specific single-chain variable fragment that blocked AFP and inhibited HCC cell growth. The results indicated that the anti-AFP single-chain variable fragment (scFv) induced growth inhibition of AFP-expressing HCC cell lines in vitro through induction of G1 cell cycle arrest and apoptosis. The mechanism of apoptosis probably involved with blocking AFP internalization and regulation of the PTEN/PI3K/Akt signaling network. Moreover, the anti-AFP-scFv also effectively sensitized the HepG2 cells to paclitaxel (PTX) at a lower concentration. The combination effect of PTX and anti-AFP-scFv displayed a synergistic effect on HepG2 cells both in vitro and in vivo. Our results demonstrated that targeting AFP by specific antibodies has potential immunotherapeutic efficacy in human HCC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据