期刊
TREE GENETICS & GENOMES
卷 12, 期 3, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s11295-016-1008-9
关键词
Tea plant; Linkage map; QTL analysis; Bud flush; Young shoot color; Leaf size
资金
- Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-23]
- Twelfth Five-Year Project of Sichuan Province for Tea Plant Breeding, Science and Technology Department Project of Sichuan Province [2012-12CGZHZX0579]
Tea plant (Camellia sinensis) is a major beverage crop across the world. To uncover the genetic controls of agronomic traits and facilitate marker-assisted breeding (MAB) in tea plant, we constructed a saturated SSR-based linkage map using an F-1 population derived from the crossing of 'Longjin43' x 'Baihaozao'. A total of 483 SSR markers, consisting of 117 novel loci, 129 transferred from other tea plant maps, and 237 previously mapped, were successfully integrated into a new consensus map. The map has 15 linkage groups, covering 1226.2 cM in total with an average marker distance of 2.5 cM. The 126 markers in common enabled us to align this map to the reference genetic maps of tea plant. Phenotype data were collected in 2014 and 2015 for five traits: timing of spring bud flush (TBF), young shoot color (YSC), mature leaf length (MLL), mature leaf width (MLW), and leaf shape index (LSI, i.e., MLL/MLW). QTL analyses were performed for the five traits using the new consensus map and 15 QTLs were identified. The SSR markers, linkage map, and QTLs reported here are useful resources for future QTL mining, identification of causal genes, and MAB in tea plant.
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